An Investigation of the Effect of Catechol Oxidase

Abstract This experiment tested the effect of enzyme parsimony on reply rate. It is evident that variables that variety around an enzyme will change the enzymes powerfulness to utilisation at its optimal level. Changing enzyme concentration will either increase the answer or rate or decrease it. This depends on how much substrate in that respect is available. If there is more enzyme accordingly substrate then when the substrate is sunk the enzyme has nothing to catalyze and so the reaction rate slows bundle. In this experiment catechol oxidase from potato was used as the enzyme and catechol as the substrate. To hear the rate of reaction, the melanoid pigment from solanum tubersome (potato juice) was mensurable by the forcefulness of its tinge. The color was measured using a spectrophotometer at an absorbance of 420 nm. The results showed that as the enzyme had more time to react, the greater the color intensity was and the greater the reaction rate. The highest abso rbance read was 0.594 at 8 minutes in test tube 3. The results were consistent in the predictions. Introduction entirely living cells carry out metabolous activities. These activities provide zippo for many other chemical reactions.

The rate of these reactions is controlled by enzymes which atomic number 18 tertiary proteins created to catalyze reactions by lowering its activation energy. every enzyme reaction involves a hint (substrate) binding to an enzymes active target. This forms the enzyme-substrate complex. pastime this, the enzyme substrate complex breaks down producing the enzyme and the products. Each enzyme functions only for a specific substra! te. The enzymes consideration must(prenominal) be complementary to the shape of the substrate or the reaction will not progeny place. Since enzymes are perfectly created to exercise certain functions. It is important that the catalytic elements in an active site are precisely positioned for their function (Benkovic and Schiffer, 2003). Each enzyme must wealthy person its optimal pH and the correct...If you indispensability to get a upright essay, order it on our website: OrderCustomPaper.com

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